Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. Cell invasion is the ability of malignant tumor cells to invade normal surrounding tissue, which contributes in large part to the significant morbidity and mortality of cancers. The CytoSelect™ Cell Migration and Invasion Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the invasive properties of cells. The kit contains twelve 8 um uncoated polycarbonate membrane inserts, and another twelve 8 um polycarbonate membrane inserts coated with an ECM gel layer in a 24-well plate. Cells must degrade the ECM layer and then move through the pores of the membrane. Invasive cells are then quantified using the provided fluorometric dye.